Since the renewal and encouragement of the Order of Permanent Diaconate throughout the entire Church by Vatican II, the Diocese of Jefferson City has ordained 150 men. Today, 84 ordained men serve throughout the diocese in the role of deacon. Included in that number are several men from other dioceses. In addition to the deacons active in the diocese, six men ordained for the Diocese of Jefferson City are serving in other dioceses. Several additional ordained deacons are in inactive or retired status but continue to serve the Church in various capacities.

The first class of 19 permanent deacons was ordained on May 7, 1977. The most recent ordination was October 13, 2019, when five men were ordained for service to the Diocese.